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SMART USE OF COMPUTER-AIDED SPERM ANALYSIS (CASA) TO CHARACTERIZE SPERM MOTION
PERREAULT, S D. SMART USE OF COMPUTER-AIDED SPERM ANALYSIS (CASA) TO CHARACTERIZE SPERM MOTION. Edition 1, Chapter 27, Robaire and Hinton (ed.), The Epididymis: From Molecules to Clinical Practice--A Comprehensive Survey of the Efferent Ducts, the Epididymis and the Vas Deferens. Plenum Press, New York, NY, , 459-471, (2002).
Computer-aided sperm analysis (CASA) has evolved over the past fifteen years to provide an objective, practical means of measuring and characterizing the velocity and parttern of sperm motion. CASA instruments use video frame-grabber boards to capture multiple images of spermatozoa as they swim across the microscope's field of view. The computer then derives a sperm track and multiple measures of sperm motion from each sperm. These can be used, singly or in combination to summarize the motion of sperm from treated and untreated rats in toxicology studies or fertile and infertile (or exposed) men in clinical or epidemiological studies. This chapter provides technical guidance on the generation and analysis of CASA data, and on the use of such data in assessing potential reproductive risks in test species (rats) and humans.