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NORMAL GENE EXPRESSION IN MALE F344 RAT NASAL TRANSITIONAL/RESPIRATORY EPITHELIUM
Hester, S D., G. B. Benavides, M. Sartor, L. Yoon, D C. Wolf, AND K. T. Morgan. NORMAL GENE EXPRESSION IN MALE F344 RAT NASAL TRANSITIONAL/RESPIRATORY EPITHELIUM. GENOMICS 285(1-2):301-310, (2001).
The nasal epithelium is an important target site for chemically-induced toxicity and carcinogenicity in rodents. Gene expression profiles were determined in order to provide normal baseline data for nasal transitional/respiratory epithelium from healthy rats. Cells lining the rat nasal passages were collected and gene expression analysis was performed using ClontechTM cDNA Rat Atlas 1.2 arrays (1185 genes). The percentages of genes within specific average expression ranges, were 4.2% at 45,000- 1000; 14.8% at 1000-200; 25.0% at 200-68; 56.0% below 68. The most highly expressed genes included those involved in phase I (e.g. cytochrome P450s) and phase II (e.g. glutathione-S-transferases) xenobiotic metabolism, bioenergetics (e.g. cytochrome oxidase), osmotic balance (e.g. Na+/K+ ATPase) and epithelial ionic homeostasis (e.g. ion channels). Such baseline data will contribute to further unnderstanding normal physiology of these cells and facilitate the interpretation of responses by the nasal epithelial cells to xenobiotic treatment or disease.