Science Inventory

HIGH BREVETOXIN CONCENTRATIONS IN GYMNODINIUM BREVE BLOOMS ALONG THE NORTHWEST FLORIDA COAST DURING 1999.

Citation:

Greene, R M., M C. Murrell, J C. Kurtz, R S. Stanley, F J. Genthner, AND C C. Walker. HIGH BREVETOXIN CONCENTRATIONS IN GYMNODINIUM BREVE BLOOMS ALONG THE NORTHWEST FLORIDA COAST DURING 1999. 54th Annual Meeting of the Phycological Society of America, San Diego, CA, 16-19 July 2000.

Description:

Blooms of the dinoflagellate Gymnodinium breve (i.e. red tides) produce brevetoxins (PbTx) that negatively impact the Gulf of Mexico ecosystem, human health, and local economics. Characterizing and predicting bloom events and their impacts requires knowledge of G. breve abundance and PbTx concentrations in the water column. We report results from a bloom that occurred during the fall and winter of 1999 in NW Florida coastal waters. Data were collected from 16 stations on 3 sampling dates (29 Sept., 9 Nov., 1 Dec.), including basic hydrography, nutrient concentrations, G. breve abundances, and brevetoxin concentrations. G. breve cells were enumerated using flow cytometry and PbTx's were isolated from seawater using dichloromethane (DCM) partitioning. Brevetoxins were quantified by HPLC-DAD using a C-18 column and an acetonitrile-water gradient elution. Literature estimates of total PbTx concentration (Pbtx's 1,2,3) of cultured and field-collected G. breve suggest a range in concentration from 7 to 17 pg cell-1. We measured total PbTx levels that greatly exceeded these values [Sept., 47-67 pg cell-1 (n=5); Nov., 59-126 pg cell-1 (n=3), Dec., 12-63 pg cell-1 (n=8)]. PbTx-2 was the predominant (67-75%) PbTx isomer found in these blooms. Pbtx-1 and PbTx-3 were found at 11-22% and ND-28% of total PbTx, respectively.

Record Details:

Record Type: DOCUMENT (PRESENTATION/PAPER)
Product Published Date: 08/03/2000
Record Last Revised: 06/21/2006
Record ID: 63509

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LABORATORY

GULF ECOLOGY DIVISION

MOLECULAR ECOLOGY BRANCH