Science Inventory

LIPID BIOMARKER ANALYSIS OF MARINE DINOFLAGELLATES

Citation:

Leblond, J. D. AND P J. Chapman. LIPID BIOMARKER ANALYSIS OF MARINE DINOFLAGELLATES. Presented at 53rd Annual Meeting of the Phycological Society of America, St. Louis, MO, 1-7 August 1999.

Description:

Many marine eukaryotic algae have been shown to possess characteristic chemotaxonomic lipid biomarkers. Dinoflagellates in particular are often characterized by the presence of sterols and pigments that are rarely found in other classes of algae. To evaluate the utility of chemical biomarkers in characterizing marine phytoplankton communities, methods were developed and applied in a survey of the solvent-extractable lipid constituents of cultures of different dinoflagellates. Open-column chromatography on silica was used to obtain principle lipid classes, which were characterized by TLC-FID. Lipid fractions were also derivatized and characterized by GC/MS analyses of free and esterified fatty acids and sterols. Photopigments were also examined by HPLC. Preliminary findings for representative species of the genera Alexandrium, Gymnodinium, Gyrodinium, Heterocapsa, and Prorocentrum will be presented.

Record Details:

Record Type: DOCUMENT (PRESENTATION/ABSTRACT)
Product Published Date: 08/07/1999
Record Last Revised: 06/06/2005
Record ID: 59778

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LABORATORY

GULF ECOLOGY DIVISION

MOLECULAR ECOLOGY BRANCH