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FREQUENCY OF ANEUPLOID SPERMATOZOA STUDIED BY MULTICOLOR FISH IN SERIAL SEMEN SAMPLES
VOZDOVA, M. AND S. D. PERREAULT. FREQUENCY OF ANEUPLOID SPERMATOZOA STUDIED BY MULTICOLOR FISH IN SERIAL SEMEN SAMPLES. Presented at European Environmental Mutagenesis Society 30th Annual Meeting, Budapest, Hungary, August 22-26, 2000.
Frequency of aneuploid spermatozoa studied by multicolor FISH in serial semen samples
M. Vozdova1, S. D. Perreault2, O. Rezacova1, D. Zudova1 , Z. Zudova3, S. G. Selevan4, J. Rubes1,5
1Veterinary Research Institute, Brno, Czech Republic; 2U.S. Environmental Protection Agency, Research Triangle Park, North Carolina, 3Regional Institute of Hygiene of South Moravia, Brno, Czech Republic; 4U.S. Environmental Protection Agency, Washington, DC; 5Institute of Experimental Medicine of Academy of Sciences of the Czech Republic
Three-probe fluorescence in situ hybridization was used to assess interindividual variation in the frequencies of aneuploidy for chromosome X, Y, and 8 in human sperm and peripheral lymphocytes. Fifteen healthy 20-year-old men were sampled on seven occasions over a two-year period. All the donors lived in the same community and were sampled at the same times. At the end of the study, slides were coded and randomized, and 10,000 spermatozoa per sample were scored.
Significant interindividual differences were observed for sex chromosome disomies and diploidies (p<0.001). Intraindividual differences were not found during the two year interval. The donors with consistently elevated sex chromosome disomes also exhibited significantly higher frequencies of sex chromosome aneuploidy in peripheral lymphocytes.
These individuals did not exhibit elevated sperm aneuploidy for other autosomes studied (8, 16, 18, 21). However, the frequency of 21 aneuploidy in spermatozoa was significantly higher than that of the other autosomes.
These data support the hypothesis that the sex chromosomes are more prone to aneuploidy in sperm as well as lymphocytes .
This work was supported by grant VaV340/1/1997 from the Czech Ministry of the Environment. This is an abstract of a proposed presentation and does not necessarily reflect EPA policy.
Record Details:Record Type: DOCUMENT (PRESENTATION/ABSTRACT)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LABORATORY
REPRODUCTIVE TOXICOLOGY DIVISION
GAMETE AND EARLY EMBRYO BIOLOGY BRANCH