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THE USE OF INTER SIMPLE SEQUENCE REPEATS (ISSR) IN DISTINGUISHING NEIGHBORING DOUGLAS-FIR TREES AS A MEANS TO IDENTIFYING TREE ROOTS WITH ABOVE-GROUND BIOMASS
Bates, M., K. J. Martin, AND P T. Rygiewicz. THE USE OF INTER SIMPLE SEQUENCE REPEATS (ISSR) IN DISTINGUISHING NEIGHBORING DOUGLAS-FIR TREES AS A MEANS TO IDENTIFYING TREE ROOTS WITH ABOVE-GROUND BIOMASS. Presented at OSU Center for Gene Research and Biotechnology Fall Retreat, Newport, OR, September 29, 2000.
We are attempting to identify specific root fragments from soil cores with individual trees. We successfully used Inter Simple Sequence Repeats (ISSR) to distinguish neighboring old-growth Douglas-fir trees from one another, while maintaining identity among each tree's parts. We found that all three primers evaluated gave reasonable discrimination among trees. We also found that fungi produced occasional bands with these primers. We are pursuing an in-depth analysis of the patterns for the plants at the field site to determine which bands are diagnostic for specific trees. We are also analyzing root sections, to get an idea whether there is good correlation between patterns above- and below-ground or whether soil microbiota obscure the patterns. We are looking into other methods that may complement the ISSR analyses, in the case of difficulty in interpreting root ISSR patterns.