Science Inventory

CONFOCAL MICROSCOPY SYSTEM PERFORMANCE: LASER POWER MEASUREMENTS

Citation:

Zucker, R M. CONFOCAL MICROSCOPY SYSTEM PERFORMANCE: LASER POWER MEASUREMENTS. MICROSCOPY TODAY 10(6):20-22, (2002).

Description:

Laser power abstract
The reliability of the confocal laser-scanning microscope (CLSM) to obtain intensity measurements and quantify fluorescence data is dependent on using a correctly aligned machine that contains a stable laser power. The laser power test appears to be one of the most useful tests to quickly evaluate if a system is misaligned or functioning sub optimally by recording insufficient laser power readings. The test using a power meter can indicate if the system is aligned properly up to the plane of excitation on the stage or if the machine has a defective component (i.e. a dying laser, or a defective fiber). In our experience, without sufficient power throughput in the system, the PMT voltages will have to be increased to high values to visualize fluorescence derived from specimens, which will introduce reduced image quality. In addition the cause of the decreased laser power may result in other problems i.e. laser instability, loss of axial resolution, and increased noise

Record Details:

Record Type: DOCUMENT (JOURNAL/NON-PEER REVIEWED JOURNAL)
Product Published Date: 11/01/2002
Record Last Revised: 12/22/2005
Record ID: 59427

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LABORATORY

REPRODUCTIVE TOXICOLOGY DIVISION

GAMETE AND EARLY EMBRYO BIOLOGY BRANCH