Citation:

Zucker, R., J. Ortenzio, L. Degn, J. Lerner, AND W. Boyes. Biophysical Comparison of Four Silver Nanoparticles Coatings using Microscopy, Hyperspectral Imaging and Flow Cytometry. PLOS ONE . Public Library of Science, San Francisco, CA, , 1-24, (2019). https://doi.org/10.1371/journal.pone.0219078

Impact/Purpose:

The selective uptake of Engineered nanoparticles by cells and tissues is important in order to determine the biological effects on cells and man. The present studies evaluated the role of surface coatings of AgNP on cellular uptake, cellular disposition, and the interactions with cellular constituents using flow cytometry side scatter, surface plasmonic resonance (SPR), microscope morphology, and hyperspectral imaging. This data will be useful for scientists to better investigate the interaction of nanoparticles with cells to have a better understanding of human risk from nanoparticles.

Description:

Four types of silver nanoparticles with different surface coatings (branched polyethyleneimine (bPEI), citrate, polyvinylpyrrolidone (PVP), and polyethylene glycol (PEG)) were compared using flow cytometry, microscopic morphology and hyperspectral imaging. One type of gold nanoparticles(PVP) was compared to the silver nanoparticles. The bPEI compound was positively charged and entered cells at a higher rate than the negatively or neutral charged compounds. The bPEI compound was also toxic to mitochondria, resulting in beaded mitochondria formations. Hyperspectral imaging showed that bPEI and citrate coated particles acquired molecules in the solution which then increased their light scattering compared to PEG and PVP coated particles which had less reactive surface coatings. Citrate and bPEI had higher surface plasmonic resonance (SPR) in unfixed cells than either PEG and PVP surface coatings. This was presumably due to aggregation of these particles inside the cell.

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