Science Inventory

In silico site-directed mutagenesis informs species-specific predictions of chemical susceptibility derived from the Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS) tool

Citation:

Doering, J., S. Lee, K. Kristiansen, L. Evenseth, M. Barron, I. Sylte, AND C. LaLone. In silico site-directed mutagenesis informs species-specific predictions of chemical susceptibility derived from the Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS) tool. SETAC Europe, Rome, ITALY, May 13 - 16, 2018.

Impact/Purpose:

This is a presentation showing that substitutions in identities of key amino acids cause no change in protein interactions with chemicals if the amino acids share the same functional properties and have comparable size. These findings were used to improve a computational tool that allows automated predictions of chemical susceptibility among species based on identities of key amino acids in their protein targets. This work supports aims of CSS AOPDD 17.01.01 Task 1.1c towards the development of tools that address challenges of species extrapolation of chemical susceptibilities to guide more objective human and ecological hazard assessments.

Description:

The Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS) tool was developed to address needs for rapid, cost effective methods of species extrapolation of chemical susceptibility. Specifically, the SeqAPASS tool compares the primary sequence (Level 1), functional domain sequence (Level 2), or individual amino acid residues at key positions (Level 3) of the protein target of a chemical in a known sensitive species to sequences of other species and calculates sequence similarity metrics to predict potential cross-species chemical susceptibility. Level 3 analyses offer the greatest resolution for extrapolation of chemical susceptibility across specific species, but uncertainties into the role of specific amino acid substitutions at key positions of proteins and whether they affect interaction with chemicals made manual interpretation of Level 3 analyses time consuming and potentially inconsistent. Therefore, this study used in silico site-directed mutagenesis coupled with docking simulations of computational models for acetylcholinesterase (AChE) and ecdysone receptor (EcR) to investigate how specific amino acid substitutions impact protein-chemical interaction. This study found that substitutions in identities of key amino acids cause no change in chemical interaction with a protein if residues share the same side chain functional properties and have comparable molecular dimensions, while differences in side chain functional properties or molecular dimensions can reduce protein-chemical interaction. These findings were considered in the development of automated Level 3 analyses and enabling automatically generated species-specific predictions of chemical susceptibility. These predictions were shown to agree with Level 1 and 2 predictions of AChE and EcR for more than 90 % of investigated species, but also identified dramatic species-specific differences in chemical susceptibility that align with results from standard toxicity tests. The consistency of automated predictions of susceptibility across Levels 1, 2 and 3 and agreement with results of standard toxicity tests provides a compelling line-of-evidence for use of SeqAPASS in deriving screening level species-specific chemical susceptibility predictions across broad taxonomic groups applicable to addressing challenges in species extrapolation for human and ecological hazard assessment.

Record Details:

Record Type: DOCUMENT (PRESENTATION/POSTER)
Product Published Date: 05/16/2018
Record Last Revised: 05/21/2018
OMB Category: Other
Record ID: 340838

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LABORATORY

MID-CONTINENT ECOLOGY DIVISION