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Assessment of the in vitro dermal irritation potential of cerium, silver, and titanium nanoparticles in a human skin equivalent model
Miyani, V. AND M. Hughes. Assessment of the in vitro dermal irritation potential of cerium, silver, and titanium nanoparticles in a human skin equivalent model. Cutaneous and Ocular Toxicology. Taylor & Francis Group, London, Uk, 36(2):145-151, (2017).
Dermal contact with nanomaterials is one route of human exposure to nanomaterials. The potential effects from nanomaterials on skin are not known. The objective of this study was to assess the potential for dermal irritation of nanomaterials using a human in vitro 3-dimensional skin model. Regulatory scientists in OPPT would have an interest in this research as there is limited information on the dermal toxicity of nanomaterials. The significance of the results show that of the metal and metal oxide nanomaterials examined in this in vitro human model, dermal irritation was not observed.
AbstractDermal exposure to metals may res·ult in irritant contact dermatitis. This study examined the potential of metal nanoparticles to elicit irritant contact dermatitis in a human skin equivalent model (HSEM) derived from epidermal keratinocytes. These cultured cells form a multi-layered,highly differentiated model of human, skin. Several Ag (10-100 nm), Ti02 (22-214 nm) and Ce02 (15-70 nm) nanoparticles were used. The Ag particles were either coated/shelled with silica or capped with citrate or polyvinylpyrrolidone. The Ag nanoparticles were dissolved in water. The Ti02 and Ce02 particles were suspended in cell culture media containing 10% fetal bovine serum. Stock suspensions of these particles were dispersed using a probe sonicator. The particles (1 mg/ml) were applied to the epidermal surface of the HSEM. Three to four wells of HSEM were tested per particle. The positive control was 5% sodium dodecyl sulfate (SDS) and the negative control was either saline or culture media. After 1 h exposure at 37 °C, the HSEM was washed with saline, and then incubated for 42 hat 37 °C, with a change of media at 24 h. Viability of the H~EM was assessed using the MTT assay. A test substance is considered an irritant if the HSEM viability is < 501Yo. The mean viability for the SDS treated HSEM was 7.8%. The viabilities of the nanoparticle treated HSEM were 91% or greater. Under the in vitroconditions used in this study, the Ag, Ti02 and Ce02 nanoparticles examined were not dermal irritants. The formed stratum corneum of the HSEM may limit penetration of metal nanoparticles to induce inflammation and cell death, or their inherent dermal irritancy potential is very low.
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LABORATORY
INTEGRATED SYSTEMS TOXICOLOGY DIVISION