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Live-cell Imaging Approaches for the Investigation of Xenobiotic-Induced Oxidant Stress
Wages, P., W. Cheng, E. Gibbs-Flournoy, AND J. Samet. Live-cell Imaging Approaches for the Investigation of Xenobiotic-Induced Oxidant Stress. Biochimica et Biophysica Acta. Elsevier Science Ltd, New York, NY, 1860(12):2802-15, (2016).
This is a review article that highlights the advantages of live cell imaging as a methodological approach in the investigation of the oxidative effects of toxicants such as environmental agents of regulatory concern.
BACKGROUND: Oxidant stress is arguably a universal feature in toxicology. Research studies on the role of oxidant stress induced by xenobiotic exposures have typically relied on the identification of damaged biomolecules using a variety of conventional biochemical and molecular techniques. However, there is increasing evidence that low-level exposure to a variety of toxicants dysregulates cellular physiology by interfering with redox-dependent processes.SCOPE OF REVIEW: The study of events involved in redox toxicology requires methodology capable of detecting transient modifications at relatively low signal strength. This article reviews the advantages of live-cell imaging for redox toxicology studies.MAJOR CONCLUSIONS: Toxicological studies with xenobiotics of supra-physiological reactivity require careful consideration when using fluorogenic sensors in order to avoid potential artifacts and false negatives. Fortunately, experiments conducted for the purpose of validating the use of these sensors in toxicological applications often yield unexpected insights into the mechanisms through which xenobiotic exposure induces oxidant stress.GENERAL SIGNIFICANCE: Live-cell imaging using a new generation of small molecule and genetically encoded fluorophores with excellent sensitivity and specificity affords unprecedented spatiotemporal resolution that is optimal for redox toxicology studies. This article is part of a Special Issue entitled Air Pollution, edited by Wenjun Ding, Andrew J.Ghio and Weidong Wu.