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High Fructose/High Fat Diets Mediate Changes in Protein Carbonyl Content in the Rat Brain With and Without Ozone Exposure
Valdez, J., J. Richards, A. Johnstone, J. Royland, AND P. Kodavanti. High Fructose/High Fat Diets Mediate Changes in Protein Carbonyl Content in the Rat Brain With and Without Ozone Exposure. Society of Toxicology Meeting, New Orleans, LA, March 13 - 17, 2016.
This abstract will be presented at the Society of Toxicology Meeting, March 13-17, 2016, New Orleans, LA.
The consumption of diets rich in fat or fructose have been correlated to a rise in type-2 diabetes and obesity. These diet-induced physiological changes have been shown previously to cause an increase in responsiveness to air pollutants such as ozone (03). 03 is a pervasive air pollutant that has been shown to not only have negative effects on the pulmonary, cardiac, and metabolic systems, but also on the central nervous system. The objective of this study was to measure 03- induced oxidative damage in brain regions of rats fed either nonnal, high fat, or high fructose diets. Thirty day old male Brown Norway rats were fed diets for 12 weeks prior to and during 03 exposure (5 hr/day, 1 day/wk, for 4 weeks). Frontal cortex (FC), cerebellum (CB), hippocampus (HIP), and hypothalamus (HYP) were dissected, quick frozen on dry ice, and stored at -80°c. Protein earbonyls (PC) were assayed using conunercial kits. Hydrogen peroxide and ferrous ammonium sulfate used as positive controls, increased PC in cortical tissue in vitro in a concentration dependent manner. There was an increase in PC due to 03 exposure in the FC of rats on the normal diet, however, this was not observed in the fructose- and fat-fed groups. In contrast, the HIP and HYP of rats on the normal diet showed a decrease in PC following 03 exposure. No other changes were noted between treatments in the HIP, but in the HYP, there was an increase in PC in the high fat diet group. Rats fed the high fat diet showed increased PC production within the CB regardless of treatment. The high fructose diet also increased PC in the CB relative to control diet following 03 exposure. These data show that dietary condition can alter the production of PC in the brain, with region specific effects following 03 exposure. (This abstracl does nor necessarily reflect USEPA policy).
Record Details:Record Type: DOCUMENT (PRESENTATION/ABSTRACT)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LABORATORY
TOXICOLOGY ASSESSMENT DIVISION