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Machine Learning Approaches for Predicting Human Skin Sensitization Hazard
Strickland, J., D. Zang, M. Paris, N. Kleinstreuer, D. Lehmann, D. Allen, J. Matheson, A. Jacobs, A. Lowit, AND W. Casey. Machine Learning Approaches for Predicting Human Skin Sensitization Hazard. American Society for Cellular and Computational Toxicology, RTP, NC, October 01 - 02, 2015.
The Interagency Coordinating Committee on the Development of Alternative Methods (ICCVAM) is composed of representatives from 15 U.S. Federal regulatory and research agencies that use toxicological safety testing information. One of the top priorities of ICCVAM is the development and evaluation of non-animal approaches to identify potential skin sensitizers. This abstract describes an ongoing effort to develop machine learning approaches for predicting human skin sensitization hazard.
One of ICCVAM’s top priorities is the development and evaluation of non-animal approaches to identify potential skin sensitizers. The complexity of biological events necessary for a substance to elicit a skin sensitization reaction suggests that no single in chemico, in vitro, or in silico method will provide a complete replacement for currently accepted animal tests. Thus, ICCVAM is evaluating machine learning approaches to integrate relevant data based on the OECD adverse outcome pathway for skin sensitization to predict human skin sensitization hazard. Models were built and tested using input variables derived from public data for 96 chemicals with human skin sensitization hazard informatioSn. Data were obtained from three in chemico or in vitro methods (direct peptide reactivity assay [DPRA], human cell line activation test [h-CLAT], and KeratinoSens assay) and six physicochemical properties (octanol:water partition coefficient [log P], water solubility, vapor pressure, molecular weight, melting point, and boiling point. A read-across prediction of skin sensitization hazard for each substance, produced using OECD QSAR Toolbox, provided an additional input. All of these data were used as potential features to predict human hazard using two machine learning approaches, support vector machine and logistic regression, applied to 12 combinations of features. Models were trained on a set of 72 substances and tested on an external set of 24 substances. The feature set containing DPRA, h-CLAT, KeratinoSens, OECD QSAR Toolbox, and log P produced the best performing model by either approach: accuracy = 99% (71/72), sensitivity = 98% (50/51), and specificity = 100% (21/21) for the training set; and accuracy = 96% (23/24), sensitivity = 93% (14/15), and specificity = 100% (9/9) for the test set. The performance of this integrated approach was better at predicting human skin sensitization hazard than the local lymph node assay or any of the in chemico, in vitro, or in silico methods alone. These data suggest that computational methods are promising tools to effectively identify potential skin sensitizers without testing in animals. This project was funded in whole or in part with Federal funds from the NIEHS, NIH under Contract No. HHSN273201500010C. This abstract does not reflect US EPA Policy.
URLs/Downloads:ORD-013254-FINAL ABSTRACT-ICCVAMSSIDS HUMAN-ASCCT 06JUL2015.DOCX
Record Details:Record Type: DOCUMENT (PRESENTATION/ABSTRACT)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LABORATORY
ENVIRONMENTAL PUBLIC HEALTH DIVISION
CARDIOPULMONARY AND IMMUNOTOXICOLOGY BRANCH