Science Inventory

Exposure of Mammalian Cells to Air-Pollutant Mixtures at the Air-Liquid Interface

Citation:

Zavala-Mendez, J., R. Greenan, B. Chorley, N. Hanley, Todd Krantz, C. King, L. Walsh, A. Ledbetter, D. DeMarini, P. White, M. Higuchi, AND Ian Gilmour. Exposure of Mammalian Cells to Air-Pollutant Mixtures at the Air-Liquid Interface. Society of Toxicology Meeting, San Diego, CA, March 23 - 27, 2015.

Impact/Purpose:

This abstract will be presented at the Society of Toxicology Meeting, March 23-27, 2015, San Diego, CA

Description:

It has been widely accepted that exposure of mammalian cells to air-pollutant mixtures at the air-liquid interface is a more realistic approach than exposing cell under submerged conditions. The VITROCELL systems, are commercially available systems for air-liquid interface exposures. The VITROCELL® 6 CF system was used to expose BEAS-2B cells to diesel exhaust (DE), smog,and ozone (03) for 1hour. Markers of cytotoxicity, inflammation,and oxidative stress were assessed at 6 and 24 hours post­ exposure. In order to use the system, extensive modifications to the aerosol delivery system were required to improve temperature and humidity control. DE particulate matter concentrations ranged from 0.5-2.0 mg/m3. No cytotoxicity or inflammation was observed, whereas levels of Hmox-1 were elevated at the highest concentrations for the 6 and 24 hours post-exposure. Cells were exposed to a simulated smog atmosphere (325 ug/m3 SOA,O.llppm 03, and 0.17ppm NOx) generated using the EPA's Mobile Reaction Chamber. For this atmosphere, significant increases in IL-8 and TNF-a were observed at 6 hours post-exposure. Finally, cells were exposed to 03 concentrations of 0.5,1, 2.5, and 5ppm. Significant cytotoxicity (measured by LDH release) and inflammation (measured by IL-6 and IL-8) was observed only at concentrations of 5 ppm. These results indicate that, with respect to the conditions examined, high concentrations of DE and 03 were needed to induce adverse effects, while lower concentrations of a reacted atmosphere were sufficient. A new in-house in vitro exposure system that accommodates 6-well and 24-well platforms is being developed to improve the delivery of both gas- and particle-phase pollutants to the cells. [Abstract does not necessarily reflect the views or policies of the U.S. EPA.]

URLs/Downloads:

ORD-011641-ABSTRACT.PDF   (PDF,NA pp, 214.851 KB,  about PDF)

Record Details:

Record Type: DOCUMENT (PRESENTATION/ABSTRACT)
Product Published Date: 03/26/2015
Record Last Revised: 04/16/2015
OMB Category: Other
Record ID: 307705

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LABORATORY

ENVIRONMENTAL PUBLIC HEALTH DIVISION

INHALATION TOXICOLOGY FACILITIES BRANCH