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Cell Signaling and Neurotoxicity: Protein Kinase C in vitro and in vivo
Citation:
KODAVANTI, PRASADA RAO S. Cell Signaling and Neurotoxicity: Protein Kinase C in vitro and in vivo. Chapter 21, In Vivo Neurotoxicology: Methods and Protocols, Methods in Molecular Biology. Springer Science + Business Media, New York, NY, 758:307-19, (2011).
Impact/Purpose:
This book chapter is on different methods available for estimating PKC activity as well as its translocation from cytosol to the membrane where it gets activated. Cell signaling molecules such as protein kinase C (PKC) have been shown to play critical roles in motor activity, development of the nervous system, and in learning and memory. PKC also has been shown to be associated with several neurological disorders including Alzheimer's disease, status epilepticus, and cerebellar ataxia. In the literature, there is abundant information linking PKC to cognitive function, long-term potentiation or brain structural changes. In addition, there is a growing concern about the effects of chemicals on the developing nervous system. Chemical exposure at critical periods of development can be associated with effects ranging from subtle to profound on the structure and/or function of the nervous system. Understanding critical biological molecular targets, that underlie chemical-induced neurotoxicity, will provide a scientific basis for risk assessment. In addition to describing the methods, we presented our previous data showing the relationship between changes in PKC (as assayed using radioactive material or by western blots) and the neurotoxic effects caused by environmental chemicals in vitro and in vivo. This information will be useful for understanding mode of action for the chemical-induced neurotoxic effects.
Description:
There is a growing concern about the effects of chemicals on the developing nervous system. Chemical exposure at critical periods of development can be associated with effects ranging from subtle to profound on the structure and/or function of the nervous system. Understanding critical biological molecular targets, that underlie chemical-induced neurotoxicity, will provide a scientific basis for risk assessment. Cell signaling molecules such as protein kinase C (PKC) have been shown to play critical roles in motor activity, development of the nervous system, and in learning and memory. PKC also has been shown to be associated with several neurological disorders including Alzheimer's disease, status epilepticus, and cerebellar ataxia. In the literature, there is abundant information linking PKC to cognitive function, long-term potentiation or brain structural changes. Here, we show the relationship between changes in PKC (as assayed using radioactive material or by western blots) and the neurotoxic effects caused by environmental chemicals in vitro and in vivo.
