Science Inventory

Phytotoxicity assay for seed production using Brassica rapa L.

Citation:

OLSZYK, D. M., T. G. PFLEEGER, E. LEE, AND M. PLOCHER. Phytotoxicity assay for seed production using Brassica rapa L. Integrated Environmental Monitoring and Assessment. SETAC Press, Pensacola, FL, 6(4):725-734, (2010).

Impact/Purpose:

Although pesticide drift can affect crop yield adversely, current plant testing protocols emphasize only the potential impacts on vegetative plant growth.

Description:

Although pesticide drift can affect crop yield adversely, current plant testing protocols emphasize only the potential impacts on vegetative plant growth. The present study was conducted to determine whether a plant species with a short life cycle, such as Brassica rapa L. Wisconsin Fast Plants®, can be used to indicate potential effects on seed production of herbicides applied at relatively low levels (e.g., low field application rates [FAR]). The effects of ≤0.1 × FAR of aminopyralid, cloransulam, glyphosate, primisulfuron, or sulfometuron applied 14 d after emergence (DAE), were evaluated for B. rapa grown in mineral soil in pots under greenhouse conditions. Effects were expressed as the effective concentration of the herbicide producing a 25% reduction in a response (EC25) based on nonlinear regression. Brassica rapa seed dry weight was reduced by sulfometuron at an EC25 of 0.00014 × a field application rate (FAR) of 53 g active ingredient (a.i.) ha−1, primisulfuron at 0.008 (experiment 1) or 0.0050 (experiment 2) × FAR of 40 g a.i. ha−1, cloransulam at 0.022 × FAR of 18 g a.i. ha−1, glyphosate at 0.0399 × FAR of 834 g a.i. ha−1, and by aminopyralid at 0.005 × FAR of 123 g a.i. ha−1, but only for 1 of 2 experiments. Reduced seed production occurred at less than the FAR that reduced shoot dry weight with sulfometuron and primisulfuron, whereas neither aminopyralid, cloransulam, nor glyphosate affected shoot dry weight. A short life cycle form of B. rapa could be used to indicate reduced seed production with plants grown only 1 week longer (∼35 DAE) than as the current vegetative vigor test for nontarget herbicide effects on plants.

URLs/Downloads:

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Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Product Published Date: 10/01/2010
Record Last Revised: 08/01/2012
OMB Category: Other
Record ID: 214668

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LABORATORY

WESTERN ECOLOGY DIVISION

ECOLOGICAL EFFECTS BRANCH