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COLLECTION AND ANALYSIS OF NON-INVASIVE BIOMARKERS OF AS EXPOSURE IN HUMANS TO SIMPLY FIELD STUDIES OF AS TOXICITY.
Citation:
ADAIR, B., V. DEVESA I PEREZ, E. E. HUDGENS, M. T. SCHMITT, M. STYBLO, R. L. CALDERON, AND D. J. THOMAS. COLLECTION AND ANALYSIS OF NON-INVASIVE BIOMARKERS OF AS EXPOSURE IN HUMANS TO SIMPLY FIELD STUDIES OF AS TOXICITY. . Presented at American Chemical Society Annual Spring Meeting, Atlanta, GA, March 26 - 30, 2006.
Description:
Measuring As metabolites in biological matrices provides information about dose- response relationships. We examined toenail and urinary total As (totAs) as biomarkers of exposure. In regressions with inorganic As in water, toenail totAs correlated (p<0.0001, r2adjusted=0.3922), but urinary totAs did not (p= 0.1369), suggesting that toenail totAs provides a useful biomarker of long term exposure. In metabolic studies, an approach is needed that preserves oxidation states of urinary arsenic metabolites. We optimized a solid phase extraction (SPE) technique separating and preserving arsenicals in urine based on oxidation state. Trials using urine reference materials spiked with multiple concentrations of AsIII and AsV species resulted in 100% binding of AsIII compounds and >90% recoveries of AsV compounds (standard deviation <10%, n=36) compared to spiked concentrations. These matrices and techniques are ideal for field studies, because arsenical stability from collection to analysis is assured. (US EPA policy is not reflected here).