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SYMBIODINIUM ISOLATES FROM STONY CORAL: ISOLATION, GROWTH CHARACTERISTICS AND EFFECTS OF UV IRRADIATION
Rogers, J E., L M. Oliver, AND L J. Hansen. SYMBIODINIUM ISOLATES FROM STONY CORAL: ISOLATION, GROWTH CHARACTERISTICS AND EFFECTS OF UV IRRADIATION. Presented at 55th Annual Phycological Society of America Meeting, Estes Park, CO, June 8-12,2001.
Symbiodinium spp. Isolates from Stony Coral: Isolation, Growth Characteristics and Effects of UV Irradiation (Abstract). J. Phycol. 37(3):42-43.
Symbiodinium species were isolated from Montipora capitata, Acropora palmata and two field samples of Porites porites. Cultures were grown and maintained on L1 medium prepared with 0.22 mm filtered seawater (34 ppt). Cultures of P. porites (JR02) contained both stationary cocoid cells, cysts and motile gymnodinoid cells. Cocoid cells ranged in size from small cells (10-12 ?m) to large cysts (23-25 ?m). Mature large cysts contained eight gymnodinoid cells. Cocoid cells of intermediate sizes contained two or four cells which ultimately divided to form the eight-gymnodinoid cells found in the large cysts. In log growth cultures, new cells were derived from the release of the eight-gymnodinoid cells. Large cocoid cysts were not often observed in mature cultures. Gymnodinoid cells were motile and became attached to the container surface where they rapidly spun as do most Symbiodinium sp. At some point the gymnodinoid cells would lose their motility and sink to the base of the container where they would convert to cocoid cells. Cultures of the other isolates contained similar cell types. A laboratory exposure system was developed and exposures to UV light were conducted in a solar simulator over a range of UV doses. Cultured Symbiodinium species demonstrated higher sensitivity to UV than their analogous coral/zooxanthellae complexes.