Science Inventory

MONITORING THE AIR FOR TOXIC AND GENOTOXIC COMPOUNDS

Citation:

Rogers, K R. MONITORING THE AIR FOR TOXIC AND GENOTOXIC COMPOUNDS. Presented at Green Chemistry Gordon Conference, Bristol, RI, July 4-9, 2004.

Impact/Purpose:

The overall objective of this task is to develop scientifically sound sampling and bioanalytical approaches for screening and monitoring of hazardous wastes. These techniques are expected to provide the Agency with improved screening and field portable methods to characterize, reduce, and control risk to human health and the environment. Specific objectives will include development and characterization of the following concepts:

SPMDs for passive accumulation of TICs

Bioassays for toxic and genotoxic compounds

MIPs for volatile and semivolatile toxic organics

Rapid screening assays using the previously listed components.

Description:

A time-integrated sampling system interfaced with a toxicity-based assay is reported for monitoring volatile toxic industrial chemicals (TICs). Semipermeable membrane devices (SPMDs) using dimethyl sulfoxide (DMSO) as the fill solvent accumulated each of 17 TICs from the vapor phase. Uptake kinetics experiments for one of these compounds (acrolein) indicated that it was significantly sequestered (i.e., 10 percent of the 24 hr maximum) in as little as 10 min and was concentrated by a factor of over 200 in 24 hr as measured using both mass and toxicity assays. The effect of each of the TICs on the Microtox bacterial luminescence assay was determined both from a direct assay and a vapor accumulation assay using SPMDs. The response of the IQ-tox assay based on Daphnia magna was also investigated for the 17 TICs. EC50 values (i.e., concentration yielding 50% inhibition of measured response) were similar to those observed for the Mocrotox assay.

Although this work was reviewed by EPA and approved for publication, it may not necessarily reflect official Agency policy.

Record Details:

Record Type: DOCUMENT (PRESENTATION/ABSTRACT)
Product Published Date: 07/07/2004
Record Last Revised: 06/21/2006
Record ID: 85108

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL EXPOSURE RESEARCH LABORATORY

HUMAN EXPOSURE AND ATMOSPHERIC SCIENCES DIVISION

EXPOSURE & DOSE RESEARCH BRANCH