Science Inventory

CAPILLARY ELECTROPHORESIS IMMUNOASSAY FOR 2,4-DICHLOROPHENOXYACETIC ACID

Citation:

Rogers, K R., A. B. Apostol, AND W C. Brumley. CAPILLARY ELECTROPHORESIS IMMUNOASSAY FOR 2,4-DICHLOROPHENOXYACETIC ACID. ANALYTICAL LETTERS 33(3):443-453, (2000).

Description:

A capillary electrophoresis (CE) immunoassay format for 2,4-dichlorophenoxyacetic acid (2,4-D) is demonstrated. A fluorescent labeled 2,4-D analog competes with the analyte of interest for a finite number of binding sites provided by anti-2,4-D monoclonal antibodies. CE then provides a means of separating and measuring both the free and antibody-bound fluorescent tracer using laser-induced fluorescence detection. For this assay format, the amount of free tracer is a sensitive indicator for the concentration of analyte present in the sample. A sequential injection format allows the rapid analysis of a small number of samples. The dynamic concentration range for 2,4-D in either buffer or river water is 5 ppb to 1000 ppb.

The U.S. Environmental Protection Agency (EPA), through its Office of Research and Development (ORD), funded the work involved in preparing this article. It has been subject to the Agency's peer review and has been approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation by the U.S. EPA.

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Product Published Date: 01/31/2000
Record Last Revised: 12/22/2005
Record ID: 64927

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL EXPOSURE RESEARCH LABORATORY

HUMAN EXPOSURE AND ATMOSPHERIC SCIENCES DIVISION

HUMAN EXPSOURE RESEARCH BRANCH