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IMPROVED SELECTIVE ELECTROCATALYTIC OXIDATION OF PHENOLS BY TYROSINASE-BASED CARBON PASTE ELECTRODE BIOSENSOR
Rogers, K R., J. Y. Becker, AND J Cembrano. IMPROVED SELECTIVE ELECTROCATALYTIC OXIDATION OF PHENOLS BY TYROSINASE-BASED CARBON PASTE ELECTRODE BIOSENSOR. ELECTROCHIMICA ACTA 45(25-26):4373-4379, (2000).
The overall objective of this task is to develop scientifically sound sampling and bioanalytical approaches for screening and monitoring of hazardous wastes. These techniques are expected to provide the Agency with improved screening and field portable methods to characterize, reduce, and control risk to human health and the environment. Specific objectives will include development and characterization of the following concepts:
SPMDs for passive accumulation of TICs
Bioassays for toxic and genotoxic compounds
MIPs for volatile and semivolatile toxic organics
Rapid screening assays using the previously listed components.
Tyrosinase-based carbon paste electrodes are evaluated with respect to the viscosity and polarity of the binder liquids. The electrodes constructed using a lower viscosity mineral oil yielded a greater response to phenol and catechol than those using a higher viscosity oil of similar chemical composition. Despite its relatively high viscosity, the use of silicon oil Si PS 086 as carbon paste binder resulted in a 2-fold increase over Si AR 200 and a 4?6 fold increase (over mineral oils) in responses to phenol, p-chlorophenol, and p-cresol. Also reported is the enhanced enzyme electrode response to phenol and catechol resulting from pre-oxidation of these substrates using a plain carbon paste electrode in a series (in-line) dual electrode configuration.
The US Environmental Protection Agency (EPA), through its Office of Research and Development (ORD), funded this research. It has been subject to the EPA's peer and administrative review and has been approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation by EPA for use.
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL EXPOSURE RESEARCH LABORATORY
HUMAN EXPOSURE AND ATMOSPHERIC SCIENCES DIVISION
HUMAN EXPSOURE RESEARCH BRANCH