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USEPA MANUAL OF METHODS FOR VIROLOGY
Williams, F P., R E. Stetler, AND R S. Safferman. USEPA MANUAL OF METHODS FOR VIROLOGY. U.S. Environmental Protection Agency, Washington, DC, EPA/600/4-84/013 (N16), 2001.
Develop sensitive techniques to detect and identify emerging human waterborne pathogenic viruses and viruses on the CCL.
Determine effectiveness of viral indicators to measure microbial quality in water matrices.
Support activities: (a) culture and distribution of mammalian cells for Agency and scientific community research needs, (b) provide operator expertise for research requiring confocal and electron microscopy, (c) glassware cleaning, sterilization and biological waste disposal for the Cincinnati EPA facility, (d) operation of infectious pathogenic suite, (e) maintenance of walk-in constant temperature rooms and (f) provide Giardia cysts.
This chapter describes procedures for the detection of coliphases in water matrices. These procedures are based on those presented in the Supplement to the 20th Edition of Standard Methods for the Examination of Water and Eastewater and EPA Methods 1601 and 1602. Two quantitative procedures and one qualitative, presence-absence procedures are presented. The procedures can be used, without supplementary methods, to assay small volumes of water (10 mL to 1L). For larger volumes (>100L), large-scale concentration methods such as described in Chapter 14 may be incorporated into the assay scheme. However, as some concentration procedures may result in appreciable loss or inactivation of coliphage, it is recommended that the suitability of any large volume concentration method be evaluated in measured recovery trials before implementation.