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TOTAL CULTURABLE VIRUS QUANTAL ASSAY
Fout, G S., D R. Dahling, AND R S. Safferman. TOTAL CULTURABLE VIRUS QUANTAL ASSAY. U.S. Environmental Protection Agency, Washington, DC, EPA/600/4-84/013, 2001.
Develop sensitive techniques to detect and identify emerging human waterborne pathogenic viruses and viruses on the CCL.
Determine effectiveness of viral indicators to measure microbial quality in water matrices.
Support activities: (a) culture and distribution of mammalian cells for Agency and scientific community research needs, (b) provide operator expertise for research requiring confocal and electron microscopy, (c) glassware cleaning, sterilization and biological waste disposal for the Cincinnati EPA facility, (d) operation of infectious pathogenic suite, (e) maintenance of walk-in constant temperature rooms and (f) provide Giardia cysts.
This chapter describes a quantal method for assaying culturable human enteric viruses from water matrices. The assay differs from the plaque assay described in Chapter 10 (December 1987 Revision) in that it is based upon the direct microscopic viewing of cells for virus-induced cytopathic effects. The quantal method can be used to assay viruses concentrated from sewages, effluents and waters using the methods described in Chapters 5, 6 and 14 (February 1999), Dahling and Wright (1986b), Fout et al. (1996) or from sludges and other solids (Chapter 7, September 1989 Revision; Fout, 1999).