You are here:
DETERMINATION OF A VETERINARY PROPHYLACTIC PHARMACEUTICAL AND ITS TRANSFORMATION PRODUCTS IN CHICKEN EXCRETA BY CAPILLARY ELECTROPHORESIS - UV AND CAPILLARY ELECTROPHORESIS - INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY
Rosal, C G., G M. Momplaisir, AND E M. Heithmar. DETERMINATION OF A VETERINARY PROPHYLACTIC PHARMACEUTICAL AND ITS TRANSFORMATION PRODUCTS IN CHICKEN EXCRETA BY CAPILLARY ELECTROPHORESIS - UV AND CAPILLARY ELECTROPHORESIS - INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY. Presented at 24th International Symposium on Capillary Chromatography and Electrophoresis, Las Vegas, NV, May 20-24, 2001.
The overall goals of the task are to apply NERL's core capability in advanced chemical science and technology for maximum benefit in estimating exposures of ecosystems and humans to chemical stressors and to identify emerging pollution concerns, in particular long-range airborne transport of contaminants. This task comprises several subtasks, each with individual objectives:
Subtask 1: screen exposures of National Park PRIMENet ecosystems to chemical stressors, identifying indications of exposure requiring further evaluation, and use these samples evaluate new analytical methods as replacements for standard methods in future assessments of ecosystem contaminant exposures.
Subtask 2: evaluate a new mercury analytical approach with superior performance on complex solid matrices such as biological tissues, and apply the approach to estimating exposure of ecosystems and humans to mercury.
Subtask 3: determine distribution patterns of chemical contaminants in the southern Sierra Nevada Range of California, investigate topographic and weather factors that may influence the distributions, and determine if a correlation exists between contaminant distributions and extirpation patterns of the mountain yellow-legged frog.
Subtask 4: provide analytical methods to measure a number of inorganic and organic arsenic species in a variety of environmental matrices, elucidate the environmental transformations undergone by organoarsenic animal-feed additives, and determine if the potential exists for substantially increased exposure of humans and aquatic organisms to arsenic.
Arsenic animal-feed additives have been extensively used in the United States for their growth- promoting and disease-controlling properties. In particular, most broiler chickens are fed roxarsone (3-nitro- 4-hydroxyphenylarsonic acid) to control coccidiosis. Disposal of the resulting arsenic-bearing wastes is currently unregulated, and they are frequently used to fertilize crop lands. Because of the high use of roxarsone in certain geographic regions, it is important that environmental fate of this compound and its transformation products be studied in order to understand their possible impacts on human health and the environment. Therefore, determination of these compounds and naturally occurring environmental inorganic and organic arsenic compounds in various media associated with the disposal of roxarsone-amended wastes is the objective of a research project at the Environmental Sciences Division. This presentation focuses on the capillary electophoresis (CE) separation of roxarsone and its transformation products in chicken excreta. Both ultraviolet (U\I) and inductively coupled plasma mass spectrometry (ICPMS) are used for detection. UV detection is not selective enough for environmental samples, but it makes
method optimization prior to coupling CE to ICPMS very rugged and time-efficient. Concurrent to the development of the CE method, we are investigating reverse-phase ion-pair high- performance liquid chromatography (HPLC) with either UV or ICPMS detection to determine roxarsone and six other arsenic compounds. HPLC separation of all the arsenic compounds requires about 30 minutes. Pavkov and Goessier (2000) used anion exchange chromatography