Citation:

Ju, C., M. Zhang, Y. Ma, J. Lu, M. Yu, H. Chen, C. Liu, Y. Gu, Y. Fu, AND Y. Duang. Genetic and Antibiotic Resistance Characteristics of Campylobacter jejuni Isolated from Diarrheal Patients, Poultry and Cattle in Shenzhen. BIOMEDICAL AND ENVIRONMENTAL SCIENCES. Zhongguo Yufang Kexue Yanjiuyuan/Chinese Academy of Preventive Medicine, Beijing, China, 31(8):579-585, (2018). https://doi.org/10.3967/bes2018.079

Impact/Purpose:

Campylobacter jejuni is the leading cause of gastroenteritis worldwide. Its antimicrobial resistance challenges the treatment effectiveness of clinical therapy. This study investigated the prevalence and antimicrobial resistance of Campylobacter isolated from poultry, cattle and diarrhea patients in Shenzhen, a major and the fastest-growing city in South China. The results may lead to understand the risk of Campylobacter jejuni in environment and food products. Regional partners, general public, local communities and academic scientists should be interested in the results.

Description:

Objective To investigate genetic and antibiotic resistance characteristics of Campylobacter jejuni (C. jejuni) isolated from Shenzhen. Methods Multilocs sequence typing and agar dilution methods were used to define the genotype and antibiotic resistance of C. jejuni, respectively. Results In total, 126 C. jejuni strains were isolated. The prevalence of C. jejuni was 5.3% in diarrheal patients. The prevalence in poultry meat (36.5%) was higher than that in cattle meat (1.1%). However, the prevalence in poultry cloacal swabs (27.0%) was lower than that in cattle stool (57.3%). Sixty-two sequence types were obtained, among which 27 of the STs and 10 alleles were previously unreported. The most frequently observed clonal complexes were ST-21 (11.9%), ST-22 (10.3%), and ST-403 (7.1%). ST-21, ST-45, ST-354, ST-403, and ST-443 complexes overlapped between isolates from patients and cattle, whereas ST-45 and ST-574 complexes overlapped between isolates from patients and poultry. All C. jejuni were resistant to at least one antibiotic. The highest resistance rate was toward ciprofloxacin (89.7%), followed by tetracycline (74.6%), and nalidixic acid (69.0%). Conclusion This is the first report of the genotypes and antibiotic resistance of C. jejuni in Shenzhen. Overlapping clonal complexes were found between isolates from patients and cattle, and between patients and poultry.

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