You are here:
Method 446.0: In Vitro Determination of Chlorophylls a, b, c + c and Pheopigments in 1 2Marine And Freshwater Algae by Visible Spectrophotometry
Arar, E. J. Method 446.0: In Vitro Determination of Chlorophylls a, b, c + c and Pheopigments in 1 2Marine And Freshwater Algae by Visible Spectrophotometry. U.S. Environmental Protection Agency, Washington, DC, EPA/600/R-15/005, 1997.
This method provides a procedure for determination of chlorophylls a (chl a), b (chl b), c + c 1 2 (chl c + c ) and pheopigments of chlorophyll a (pheo a) 1 2 found in marine and freshwater phytoplankton. Chlorophyllide a is determined as chl a. Visible wavelength spectrophotometry is used to measure the pigments in sub-parts per million (ppm) concentrations. The trichromatic equations of Jeffrey and Humphrey(1) are used to calculate the concentrations of chl a, chl b, and chl c +c . Modified monochromatic equations of 1 2 Lorenzen(2) are used to calculate pheopigment-corrected chl a and pheo a.
Record Details:Record Type: DOCUMENT (PUBLISHED REPORT/GUIDANCE DOCUMENT)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL EXPOSURE RESEARCH LABORATORY
MICROBIOLOGICAL AND CHEMICAL EXPOSURE ASSESSMENT DIVISION