Science Inventory

Method 447.0 - Determination of Chlorophylls a and b and Identification of Other Pigments of Interest in Marine and Freshwater Algae Using High Performance Liquid Chromatography with Visible Wavelength Detection

Citation:

Arar, E. J. Method 447.0 - Determination of Chlorophylls a and b and Identification of Other Pigments of Interest in Marine and Freshwater Algae Using High Performance Liquid Chromatography with Visible Wavelength Detection. U.S. Environmental Protection Agency, Washington, DC, 1997.

Impact/Purpose:

see description

Description:

This method provides a procedure for determination of chlorophylls a (chl a) and b (chl b) found in marine and freshwater phytoplankton. Reversed phase high performance liquid chromatography (HPLC) with detection at 440 nm is used to separate the pigments from a complex pigment mixture and measure them in the sub-microgram range. For additional reference, other taxonomically important yet commercially unavailable pigments of interest are identified by retention time.

URLs/Downloads:

Method 447.0   (PDF,NA pp, 93 KB,  about PDF)

Record Details:

Record Type: DOCUMENT (ASSESSMENT DOCUMENT)
Product Published Date: 09/01/1997
Record Last Revised: 09/24/2015
OMB Category: Other
Record ID: 309414

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL EXPOSURE RESEARCH LABORATORY

MICROBIOLOGICAL AND CHEMICAL EXPOSURE ASSESSMENT DIVISION