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Metabolomic Analysis of Liver Cells Exposed to Carbon Nanotubes and Graphene Oxide
Henderson, Matt, D. Bouchard, X. Chang, I. Chowdhury, B. Foster, S. Aronson, AND Q. Teng. Metabolomic Analysis of Liver Cells Exposed to Carbon Nanotubes and Graphene Oxide. Presented at Society of Toxicology, Santonio, TX, March 10 - 14, 2013.
Poster presented athe the Society of Toxicology Annual Meeting 2013, San Antonio, TX March 10-14, 2013
Carbon nanotubes (CNTs) and other graphenic nanomaterials are being used extensively in industrial, consumer, and mechanical applications based in part on their unique structural, optical and electronic properties. Due to the widespread use of these nanoparticles (NPs), human and ecological exposure is probable and inevitable. To determine the effects CNTs and graphene oxide (GO) have on biochemical processes, metabolomics-based profiling of human (C3A) and zebrafish (ZFL) liver cells was utilized. Cell cultures were exposed to 0, 10, or 100 ng/mL of covalently or non-covalently modified nanomaterial for 24 and 48 hrs while particle size distribution, charge, and aggregation kinetics were monitored concurrent with exposure studies. Following NP exposure, metabolites were extracted and derivatized prior to GC/MS analysis or lyophilized and buffered for 1H NMR analysis. Acquired spectra and chromatograms were subjected to multivariate analysis to determine the consequence of NP exposure on the metabolite profile of C3A and ZFL cells. The resulting scores plots illustrated temporal and dose dependent responses to all classes of NPs tested. Loadings plots coupled with univariate analysis were then used to identify metabolites of interest. Preliminary data suggest that CNT and GO exposure causes perturbations in processes involved in cellular oxidation as well as fluxes in lipid metabolism and fatty acid synthesis. Dose-response trajectories are apparent for each nanomaterial tested and spectral components related to both dose and NP modifications were determined. Correlations of the significant changes in metabolites will aid in identifying potential biomarkers associated with carbonaceous nanoparticle exposure in both humans and ecologically relevant species.