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Effects of prolonged chlorine exposures upon PCR detection of Helicobacter pylori DNA.
Citation:
LYE, D. J. AND J. Acosta. Effects of prolonged chlorine exposures upon PCR detection of Helicobacter pylori DNA. CURRENT MICROBIOLOGY. Springer, New York, NY, 62(3):727-732, (2011).
Impact/Purpose:
1. Develop procedures addressing dose-response studies, susceptibility, virulence studies, etc. in H. pylori and Aeromonas.. 2. Compare virulence of environmental isolates with clinical isolates.
Description:
The effect of low doses of free chlorine on the detection by qPCR of Helicobacter pylori (H. pylori) cells by qPCR in tap water was monitored. H. pylori target sequences (within suspended, intact cells at densities of 102 to 103 cells /ml) were rendered undetectable by qPCR analysis after 17 hours of continuous exposure to low chlorine levels common to treated drinking water distribution systems. This study suggests that degradation of H. pylori DNA target sequences by chlorine levels commonly found within treated water distribution systems occurs within the average water retention times (2-3 days) commonly found in these systems.