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A FLUORESCENCE BASED ASSAY FOR DNA DAMAGE INDUCED BY TOXIC INDUSTRIAL CHEMICALS
KAILASAM, S. AND K. R. ROGERS. A FLUORESCENCE BASED ASSAY FOR DNA DAMAGE INDUCED BY TOXIC INDUSTRIAL CHEMICALS. Presented at American Chemical Society Meeting and Exposition, Washington, DC, August 28 - September 01, 2005.
The overall objective of this task is to develop scientifically sound sampling and bioanalytical approaches for screening and monitoring of hazardous wastes. These techniques are expected to provide the Agency with improved screening and field portable methods to characterize, reduce, and control risk to human health and the environment. Specific objectives will include development and characterization of the following concepts:
SPMDs for passive accumulation of TICs
Bioassays for toxic and genotoxic compounds
MIPs for volatile and semivolatile toxic organics
Rapid screening assays using the previously listed components.
One of the reported effects for exposure to many of the toxic industrial chemicals is DNA damage. The present study describes a simple, rapid and innovative assay to detect DNA damage resulting from exposure of surrogate DNA to toxic industrial chemicals (acrolein, allylamine, chloroacetone, formaldehyde, acrylonitrile, bromoethane, and crotonaldehyde) and nanomaterial (Buckminster fullerene). The assay is based on changes in the melting and annealing behavior observed for damaged DNA, which is monitored using a fluorescence indicator dye, PicoGreen .