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GENETIC POLYMORPHISM IN GYMNODINIUM GALATHEANUM CHLOROPLAST DNA SEQUENCES AND DEVELOPMENT OF A MOLECULAR DETECTION ASSAY. (R827084)
Tengs, T., H. Bowers, A. Ziman, D. Stoecker, AND D. Oldach. GENETIC POLYMORPHISM IN GYMNODINIUM GALATHEANUM CHLOROPLAST DNA SEQUENCES AND DEVELOPMENT OF A MOLECULAR DETECTION ASSAY. (R827084). MOLECULAR ECOLOGY. American Chemical Society, Washington, DC, 10(2):515-523, (2001).
Nuclear and chloroplast-encoded small subunit ribosomal DNA sequences were obtained
from several strains of the toxic dinoflagellate Gymnodinium galatheanum. Phylogenetic analyses and
comparison of sequences indicate that the chloroplast sequences show a higher degree of sequence
divergence than the nuclear homologue. The chloroplast sequences were chosen as targets for the
development of a 5'-3' exonuclease assay for detection of the organism. The assay has a very high
degree of specificity and has been used to screen environmental water samples from a fish farm
where the presence of this dinoflagellate species has previously been associated with fish kills.
Various hypotheses for the derived nature of the chloroplast sequences are discussed, as well as
what is known about the toxicity of the species. (45 References)
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL CENTER FOR ENVIRONMENTAL RESEARCH