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Developing Reference Chemical Lists for Validation of In Vitro Assays
Citation:
Judson, R. Developing Reference Chemical Lists for Validation of In Vitro Assays. Presented at OECD Working Party Extended Advisory Group for Molecular Screening and Toxicogenomics (EAGMST), Paris, N/A, FRANCE, June 17 - 21, 2019. https://doi.org/10.23645/epacomptox.8856002
Impact/Purpose:
Presentation to the Organization for Economic Co-operation and Development (OECD) Extended Advisory Group for Molecular Screening and Toxicogenomics (EAGMST) summer meeting June 2019 in Paris France.
Description:
There is an increasing desire to use new approach methods (NAMs) for chemical hazard and dose response characterization. Many in vitro assays are available, but few have been validated for use in regulatory decisions. Key to validation is the availability of reference chemicals. For high-throughput in vitro assays, multiple reference chemicals should be used, covering: varying chemical classes that are active and inactive, a range of potencies, and chemicals that are likely to cause false positive results due to assay interference. Currently, only a few in vitro assays of regulatory-relevance have more than one or two accepted reference chemicals (e.g., estrogen and androgen receptors, skin sensitization, genotoxicity). A more rapid, semi-automated approach to developing reference chemical lists is desirable. The views expressed in this presentation are those of the author and do not necessarily reflect the views or policies of the US EPA.
URLs/Downloads:
DOI: Developing Reference Chemical Lists for Validation of In Vitro Assays
JUDSON OECD REFERENCE CHEMICALS 2019-06-21.PDF (PDF, NA pp, 487.549 KB, about PDF)