Science Inventory

Metabolism Retrofit Strategies for ToxCast Assays (BOSC)

Citation:

DeGroot, D., R. Thomas, AND Steve Simmons. Metabolism Retrofit Strategies for ToxCast Assays (BOSC). Presented at BOSC CSS Meeting, RTP, NC, November 16 - 18, 2016. https://doi.org/10.23645/epacomptox.5179093

Impact/Purpose:

Poster for CSS BOSC meeting in RTP, NC.

Description:

The EPA’s ToxCast program utilizes a wide variety of high-throughput screening assays (HTS) to assess chemical perturbations of molecular and cellular endpoints. A limitation of many HTS assays used for toxicity assessment is the lack of xenobiotic metabolism (XM) which precludes the detoxification as well as toxic bioactivation of chemicals tested in vitro, thereby potentially mischaracterizing the hazard posed by these chemicals. To address this deficiency, we are pursuing two parallel approaches to retrofit HTS assays with XM. The first approach generates metabolites in the assay matrix (buffer/medium) in a cell-free manner and models the effects of circulating metabolites. The second approach uses a mix of human cytochrome P450 (CYP) mRNAs to generate metabolites within test cells and model the direct effect of proximal metabolites.

URLs/Downloads:

https://doi.org/10.23645/epacomptox.5179093   Exit

CSS_BOSC_METABOLISM_FINAL.PDF   (PDF,NA pp, 1609.846 KB,  about PDF)

Record Details:

Record Type: DOCUMENT (PRESENTATION/POSTER)
Product Published Date: 11/18/2016
Record Last Revised: 02/12/2018
OMB Category: Other
Record ID: 338799

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL CENTER FOR COMPUTATIONAL TOXICOLOGY