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Method: Isolation of Total RNA with the Life Technologies PureLink Kit
Citation:
McCullough, S. AND N. McNabb. Method: Isolation of Total RNA with the Life Technologies PureLink Kit. Nature Publishing Group, London, Uk, 2020. https://doi.org/10.21203/rs.2.21222/v1
Impact/Purpose:
This method is being made publicly available via the Nature Research Protocol Exchange to support published EPA research and ensure transparency and reproducibility.
Description:
This protocol describes the use of the Life Technologies PureLink RNA isolation kit for the isolation and purification of total RNA from cells grown in culture. Notes describing specific modifications for use with the Trans-Epithelial Exposure Model are included. Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.