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Development and validation of analytical methods for measuring diverse cyanotoxins in complex matrices using automated solid phase extraction, isotope dilution LC-MS/MS
Citation:
Sanan, T., J. Lazorchak, S. Goodrich, J. Fischer, AND D. Sundaravadivelu. Development and validation of analytical methods for measuring diverse cyanotoxins in complex matrices using automated solid phase extraction, isotope dilution LC-MS/MS. Presented at SETAC North America 44th Annual Meeting, Louisville, KY, November 12 - 16, 2023.
Impact/Purpose:
This is a presentation for a poster being submitted to the SETAC North America meeting on research under SSWR 4.04.1 related to development of analytical methods for measuring cyanotoxins.
Description:
The analysis of cyanotoxins in various matrices is complicated by challenges in extraction, concentration, and quantification. Common cyanotoxins in the United States include microcystins, anatoxins, and cylindrospermopsins, which across the various congeners have considerable differences in hydrophilicity, complicating efforts to analyze for many toxins in a single extraction/analysis workflow. Various approaches have been developed to address this challenge, including optimization of extraction conditions for individual toxin classes, and the use of chemical derivatization techniques to convert target compounds to more readily extracted products (e.g. the MMPB derivatization for microcystins). These approaches can increase the cost and time required for sample processing and analysis. Here are described efforts to develop and validate a multitoxin analytical method suitable for measurement of various microcystins, anatoxins, and cylindrospermopsin variants compatible with various matrices including tissues and soils/sediments. Automated sample concentration using online solid phase extraction is used to simplify sample preparation and reduce method detection limits, and isotopically labeled analogs of toxins are used to monitor and track method performance using isotope dilution. Details and results of the method development and validation will be presented, as well as applications to matrices including fish tissues, macroinvertebrates, zooplankton, and benthic samples.