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HAB Dynamics as Determined by Nucleic Acid Based Techniques
Citation:
SantoDomingo, J. HAB Dynamics as Determined by Nucleic Acid Based Techniques. Small drinking water systems monthly seminar, Cincinnati, OH, May 30, 2023.
Impact/Purpose:
The goal of this presentation is to provide managers, engineers, and scientists involved in small drinking water system a summary of the work we are conducting on the detection of cyanobacterial blooms in freshwater systems. The primary focus is on the use of molecular tools that circumvent some of the shortcomings of conventional approaches used to assess blooms.
Description:
In this presentation I will discuss the molecular monitoring approaches used in applied environmental microbiology studies conducted in our laboratories. The presentation will primarily focus on the detection and quantification of cyanobacterial groups and cyanotoxin genes implicated in harmful algal blooms. Results from next generation sequence analysis and qPCR/RT-qPCR methods used to characterize toxic cyanobacterial populations, associated bacterial communities, and geographically localized genotypes (strains) will be discussed. We will show how we can monitor functional genes associated with nutrients (i.e., nitrogen cycle) to detect cyanobacteria and potentially predict early warning of toxin production in environmental settings. Examples of other environmental and engineering research studies using molecular tools will also be discussed.