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Use of molecular methods to determine the presence of toxic cyanobacteria in an urban lake, Toledo
Citation:
SantoDomingo, J. Use of molecular methods to determine the presence of toxic cyanobacteria in an urban lake, Toledo. University of Toledo, Department of Chemical Engineering, Toledo, OH, March 02, 2023.
Impact/Purpose:
In this presentation I will provide examples on the use of molecular methods to study environmental and engineered microbial communities
Description:
In this presentation I will discuss the molecular monitoring approaches used in applied environmental microbiology studies conducted in our laboratories. The presentation will primarily focus on the detection and quantification of cyanobacterial groups and cyanotoxin genes implicated in harmful algal blooms. Results from next generation sequence analysis and qPCR/RT-qPCR methods used to characterize toxic cyanobacterial populations, associated bacterial communities, and geographically localized genotypes (strains) will be discussed. We will show how we can monitor functional genes associated with nutrients (i.e., nitrogen cycle) to detect cyanobacteria and potentially predict early warning of toxin production in environmental settings. Examples of other environmental and engineering research studies using molecular tools will also be discussed.