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Toxicity of Microcystin Using Culture Lysates and Testing Temperature Effects on the Toxicity of Algaecide – Hydrogen Peroxide?Toxicity of Microcystin Using Culture Lysates and Testing Temperature Effects on the Toxicity of Algaecide – Hydrogen Peroxide?Toxicity of Microcystin Using Culture Lysates and Testing Temperature Effects on the Toxicity of Algaecide – Hydrogen Peroxide
Citation:
Lazorchak, J. AND S. Goodrich. Toxicity of Microcystin Using Culture Lysates and Testing Temperature Effects on the Toxicity of Algaecide – Hydrogen Peroxide?Toxicity of Microcystin Using Culture Lysates and Testing Temperature Effects on the Toxicity of Algaecide – Hydrogen Peroxide?Toxicity of Microcystin Using Culture Lysates and Testing Temperature Effects on the Toxicity of Algaecide – Hydrogen Peroxide. UC Chemistry and Environmental Engineering Graduate Seminar, Cincinnati, OH, October 28, 2022.
Impact/Purpose:
Presentation on current status of acute and chronic toxicity of microcystin and M.S. thesis on the toxicity of Hydrogen peroxide at 2 different temperatures.
Description:
What are Harmful Algal Blooms (HABs)? Most species of algae are not harmful, but sometimes certain types bloom in excessive amounts and can cause harm to health and economies. HABs, include both algae and cyanobacteria that can produce toxins and other irritants in freshwater. Cyanobacteria—sometimes referred to as blue-green algae—have the potential to release cyanotoxins, the most common in the US being microcystins, cylindrospermopsins, anatoxins, and saxitoxins. Even though they are classified as bacteria, cyanobacteria exhibit characteristics of algae and are therefore associated with HABs. More info on EPA HABs research at: https://www.epa.gov/water-research/harmful-algal-blooms-and-cyanobacteria-research#:~:text=Commonly%20called%20harmful%20algal%20blooms,and%20other%20irritants%20in%20freshwater. EPA Standard Aquatic Toxicity Test Methods Ambient Water Quality Criteria: EPA bases aquatic life criteria on how much of a chemical can be present in surface water before it is likely to harm plant and animal life. EPA designs aquatic life criteria to protect both freshwater and saltwater organisms from short-term and long-term exposure. https://www.epa.gov/wqc/aquatic-life-criteria-and-methods-toxics Whole Effluent and Receiving Water Toxicity Testing (WET) : refers to the aggregate toxic effect to aquatic organisms from all pollutants contained in a facility's wastewater (effluent) or receiving water. Freshwater test methods have been promulgated for acute and chronic testing: Ceriodaphnia dubia – Zooplankton organism 48-hr acute, 7-day Chronic Daphnia magna – Zooplankton organism – 48-hr acute, 4-day short-term growth Pimephales promelas – Fathead minnow – 48-96-hr acute, 7-day short-term growth Neocloeon triangulifer - Mayfly – 48-hr acute, 7-day short-term growth https://www.epa.gov/cwa-methods/whole-effluent-toxicity-methods There is a lack of information to estimate safe levels for aquatic life concerning the toxicity of natural toxins produced by cyanobacteria. Given the uncertainty of standards for toxins, the cost of using them to conduct acute and chronic toxicity tests and their potential impurities, an approach is proposed using cultures of cyanobacteria and algae to generate toxins. In this study we have developed laboratory cultures of toxin producers Microcystis aeruginosa Lab Cultured Environmental Strain (microcystin) Aphanizomenon flos-aquae PCC7905 (cylindrospermopsin) Dolichospermum circinale CS-337/01 (saxitoxin). The goal was to produce toxin concentrations within +/- 20%.
