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Linkages between ColilertTM and EPA Method C qPCR results at non-Michigan Great Lakes region beach
Citation:
Hertel, S., L. Wymer, R. Haugland, L. Chapin, G. Kleinheinz, J. Creekmur, J. Kinzelman, J. lavender Braun, AND J. Seikel. Linkages between ColilertTM and EPA Method C qPCR results at non-Michigan Great Lakes region beach. 2022 Great Lakes Beach Conference, Muskegon, MI, October 31 - November 02, 2022.
Impact/Purpose:
Inform public health officials and scientists on the current status of EPA efforts to support implementation of rapid recreational beach monitoring by quantitative PCR methods in Great Lakes region (EPA Region 5).
Description:
The State of Michigan has adopted a Beach Notification Value for EPA Draft Method C based on observed relationships between results from this E. coli qPCR method and the state-approved ColilertTM culture method. Other Great Lakes region authorities may be interested in knowing whether the relationships between Method C and Colilert are similar for their beaches. Colilert and Method C results are being compared from analyses of water samples collected from Great Lakes and inland beach sites in Wisconsin, Indiana, and Ohio. As in Michigan, ordinary least squares regression analyses of paired quantitative data are being used to determine correlation between methods. Method C results from local labs are also being compared to an EPA laboratory.
