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Applying DNA Metabarcoding to Periphyton Biomonitoring: Lessons Learned from Mesocosm, Watershed, and National Scale Research
Citation:
Pilgrim, E. Applying DNA Metabarcoding to Periphyton Biomonitoring: Lessons Learned from Mesocosm, Watershed, and National Scale Research. 2019 Coastal & Estuarine Research Federation Conference, Mobile, AL, November 03 - 07, 2019.
Impact/Purpose:
Presented at the 25th Biennial Conference of the Coastal & Estuarine Research Federation in Mobile, AL, Nov 3-7.
Description:
Periphyton, especially diatoms and other algae, are increasingly being used as bioassessment tools, particularly for measuring the effects of nutrient enrichment caused by human activities in watersheds. Their use in bioassessment indices depends on accurate identification and enumeration, which has associated challenges in terms of cost, time, and effort. To help with these taxonomic challenges, we have applied multiple DNA metabarcoding techniques and statistical analyses to help identify important components of the periphyton community that could be used as indicators of nutrient effects on aquatic ecosystem conditions. These studies have involved mesocosm experiments, temporal studies within a watershed, and analyses of national survey samples, and have generated data on communities of prokaryotes, diatoms, and eukaryotes in general. We will discuss our results from relevant studies and outline lessons learned that can be applied to periphyton communities in any aquatic habitat.