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SeqAPASS User Guide v5.0
Citation:
LaLone, C. SeqAPASS User Guide v5.0. U.S. Environmental Protection Agency, Washington, DC, EPA/600/R-20/408, 2021.
Impact/Purpose:
The SeqAPASS v5.0 T tool allows users to compare protein similarity across species. This is important because chemicals act on protein targets. Therefore, by understanding how similar proteins are across species, the SeqAPASS tool can produce chemical susceptibility predictions across hundreds of species rapidly.
Description:
The US Environmental Protection Agency Sequence Alignment to Predict Across Species Susceptibility tool (SeqAPASS; https://seqapass.epa.gov/seqapass/) was developed to comparatively evaluate protein sequence and structural similarity across species as a means to extrapolate toxicity data/knowledge. SeqAPASS output provides a prediction of chemical susceptibility based on principles of evolutionary biology in understanding species relatedness at the molecular level. New to SeqAPASS Version 5 include the following: • SeqAPASS Level 3 individual amino acid comparisons now have a customizable heat map visualization for rapid interpretation of the data and utility for publication and presentation quality graphics. • In order to synthesize SeqAPASS results (i.e., data tables and visualizations) across all Levels (1, 2, and 3) of the SeqAPASS evaluation, a customizable Decision Summary Report has been created. Users can now push SeqAPASS results to a final downloadable (.pdf) summary report.
URLs/Downloads:
SEQAPASS USER GUIDE V5.0.PDF (PDF, NA pp, 10342.201 KB, about PDF)https://www.epa.gov/chemical-research/seqapass-user-guide