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Differential Isoform Usage in Fathead Minnows After Estrogenic Exposure
Citation:
Fetke, J., J. Martinson, AND A. Biales. Differential Isoform Usage in Fathead Minnows After Estrogenic Exposure. SETAC, Pittsburgh, PA, November 13 - 17, 2022. https://doi.org/10.23645/epacomptox.21445026
Impact/Purpose:
The study investigates isoform switching to gain a better understanding of how isoform usage in fish exposed to exogenous estrogen may differ with tissue type, sex, and estrogen exposure. Isoform usage is important for many biological processes and isoform switching is implicated in diseases such as cancer.
Description:
The ability to produce different transcripts from the same gene through alternative splicing, known as isoform switching, is important for dynamic regulation of gene expression. Differential isoform usage allows for greater functional diversity of the genome, and aberrant isoform switching is associated with disease such as cancer. Gene expression response to estrogen exposure have been thoroughly described in fish, however little is known about isoform expression profiles for genes responsive to estrogen exposure. This study examines isoform usage in brain and liver tissues from 8-10 month-old male fathead minnows (Pimephales promelas) after a 48h exposure to either nominal 2.5 ng/L or 10 ng/L of 17α-ethynylestradiol (EE2) as well as untreated female fathead minnows. We used RNAseq data generated from brain and liver tissues and the software package, RSEM, for quantification of isoform abundance for the purpose of characterizing isoform usage across tissues, sex, and treatment. The identification of alternative splicing events indicates differential isoform usage as a potential mediator of organismal response to chemical exposure.
URLs/Downloads:
DOI: Differential Isoform Usage in Fathead Minnows After Estrogenic Exposure
ISOFORMUSAGE_SETAC2022.PDF (PDF, NA pp, 614.8 KB, about PDF)