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Solid-phase Microextraction of Perfluorinated Compounds in Biological Matrices
Citation:
Brennan, A. AND J. Ford. Solid-phase Microextraction of Perfluorinated Compounds in Biological Matrices. The Society of Environmental Toxicology and Chemistry (SETAC) North America 41st Annual Meeting, Fort Worth, Texas, November 15 - 19, 2020. https://doi.org/10.23645/epacomptox.19341890
Impact/Purpose:
Poster presented to the Society of Environmental Toxicology and Chemistry (SETAC) North America annual meeting November 2020
Description:
Measurements of non-ionic perfluorinated compounds (PFCs) in biological matrices, including plasma, are needed for accurate determination of internal dose. Traditional sample preparation techniques for extracting these target compounds from plasma are time consuming and could result in lower recoveries for the more volatile PFCs. For comparison and possible performance improvements, liquid-liquid extraction (LLE) will be compared to solid-phase microextraction-GC/mass spectrometry (MS) techniques for extraction of target compounds from plasma. Other non-ionic PFCs have been successfully extracted from water and sediment samples using headspace-solid-phase microextraction (SPME) resulting in reduced sample preparation time and matrix interferences during quantitation compared to traditional techniques. Extending high throughput SPME techniques to biological matrices for PFCs could result in similar improvements and decreased detection limits. Initially, both headspace and direct-immersion SPME will be assessed to determine which technique yields the highest extraction efficiency for the target compounds. After selection of the specific SPME technique, SPME parameters such as fiber coating, phase volume, extraction temperature, extraction time, addition of organic additives, and GC desorption temperature and time will be optimized using standards spiked into blank plasma matrix. A final side-by-side comparison of LLE and the optimized SPME technique for the target compounds will be conducted with spiked plasma matrix for at least 3 concentrations and 7 replicates. Results from this study will include accuracy, precision, detection limits, and signal to noise ratios for each compound and method. If the SPME technique shows promise in these proof of concept studies, additional extension of SPME techniques to biological matrices other than plasma could be investigated. This abstract does not necessarily represent the views or policies of the US EPA.
URLs/Downloads:
DOI: Solid-phase Microextraction of Perfluorinated Compounds in Biological Matrices
BRENNANAA SETAC POSTER 2020 FORT WORTH TX FINAL.PDF (PDF, NA pp, 494.833 KB, about PDF)