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Genomics in formalin-fixed paraffin embedded tissue (FFPE) samples for quantitative risk assessment
Citation:
Wehmas, L., S. Hester, AND C. Wood. Genomics in formalin-fixed paraffin embedded tissue (FFPE) samples for quantitative risk assessment. HESI eSTAR Webinar, RTP, NC, May 26, 2020. https://doi.org/10.23645/epacomptox.18162857
Impact/Purpose:
Archival samples represent a great resource for understanding adverse chemical effects. Use of formalin-fixed paraffin-embedded (FFPE) tissue samples for gene expression has been limited by quality issues. Research efforts at the EPA and in collaboration with the Health and Environmental Sciences Institute's Emerging Systems Toxicology and Assessment of Risk group have developed new methods to overcome these limitation. These efforts have focused on using FFPE to better understand adverse effects of chemical exposure in a dose responsive way using existing toxicology samples. For many research studies, FFPE samples represent the only remaining biological material. The efforts presented in this webinar offer access to these archives to help advance chemical risk assessments.
Description:
Archival samples represent a vast resource for identification of chemical and pharmaceutical targets. However, use of formalin-fixed paraffin-embedded (FFPE) tissue samples in genomics has been hindered by nucleic acid damage, potential fixation related artifacts, and wide variation in sample quality. Over the past five years, the HESI eSTAR FFPE working group has identified new methodologies and harnessed advances in technology to overcome these limitations for characterizing chemical-mediated adverse outcomes in a quantitative and predictive manner. Recent work has been translated to improving mutational analysis in clinical FFPE samples and applied to determining transcriptomic-based points of departure due to chemical exposure that are indicative of later adverse histopathological effects using tissue from very old banked toxicologic samples. For many research studies, FFPE samples represent the only remaining biological material. The efforts presented in this webinar offer unprecedented access to these archives for developing quantifiable transcriptomic signatures that inform chemical mode of action and disease detection, thereby, helping advance chemical and pharmaceutical risk assessments.
URLs/Downloads:
DOI: Genomics in formalin-fixed paraffin embedded tissue (FFPE) samples for quantitative risk assessment
WEHMAS_HESI_FFPE_WEBINAR_2020V4.PDF (PDF, NA pp, 4503.834 KB, about PDF)