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Characterization of Novel Human Immortalized Thyroid Follicular Epithelial Cell Lines (SOT 2021)
Citation:
Hopperstad, K., T. Truschel, T. Wahlicht, W. Stewart, A. Eicher, T. May, AND C. Deisenroth. Characterization of Novel Human Immortalized Thyroid Follicular Epithelial Cell Lines (SOT 2021). Virtual 2021 Society of Toxicology Annual Meeting, RTP, NC, March 12 - 26, 2021. https://doi.org/10.23645/epacomptox.14470671
Impact/Purpose:
Abstract submitted to the Society of Toxicology annual meeting March 2021
Description:
Investigation of normal human thyroid function using in vitro culture systems is dependent on cells that recapitulate physiology of differentiated thyrocytes. Primary thyrocytes retain features of the native organ but have limited lifespan in culture. Immortalized thyrocytes offer an alternative if challenges with phenotypic stability can be overcome to retain functional features of primary cells. CI-SCREEN immortalization technology was applied to normal human thyroid tissue to generate four human thyroid endothelial cell (huThyrEC) line variants. The lines were characterized for transgene integration, biomarker expression, genomic stability, and proliferation rates. Thyroid Stimulating Hormone (TSH)-dependent morphology, thyroglobulin production, thyroxine hormone synthesis, and viability were assessed using conventional 2D monolayer and 3D microtissue culture formats in huThyrEC or h7H medium. Despite differential transgene profiles, the lines had similar biomarker expression patterns. TSH-dependent thyroglobulin expression was more significant for several lines in h7H than huThyrEC medium in 2D models. No thyroxine synthesis or changes in viability were noted. Comparatively, TSH-dependent thyroglobulin induction was greater for huThyrEC lines in h7H medium in 3D culture. Synthesis of thyroxine in one cell line was higher than background with TSH exposure, but not different than control. Immortalization of primary human thyrocytes yielded transgenic lines of epithelial origin. When evaluated in 2D or 3D culture formats, h7H medium supported thyroglobulin production to a greater magnitude than huThyrEC medium. One huThyrEC line cultured in 3D microtissue format marginally recapitulated T4 synthesis under continuous TSH exposure. Select human huThyrEC cell lines exhibit morphological and functional features of primary thyrocytes and are a novel resource for in vitro disease modeling and toxicity testing. The views expressed are those of the author and do not necessarily reflect the views or policies of the U.S. Environmental Protection Agency.
URLs/Downloads:
DOI: Characterization of Novel Human Immortalized Thyroid Follicular Epithelial Cell Lines (SOT 2021)
HOPPERSTAD_SOT_THYROID_POSTER_V6.PDF (PDF, NA pp, 1105.594 KB, about PDF)