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High-Throughput Transcriptomics (HTTr) Screening as a Component of NAMs-based Tiered Hazard Evaluation
Citation:
Harrill, J. High-Throughput Transcriptomics (HTTr) Screening as a Component of NAMs-based Tiered Hazard Evaluation. US EPA / Japan Ministry of the Environment 14th Bilateral Meeting on Endocrine Disruption Test Methods Development, NA (Virtual), NA (Virtual), June 09 - 10, 2021. https://doi.org/10.23645/epacomptox.16688923
Impact/Purpose:
The US EPA Center for Computational Toxicology and Exposure has research programs focused on developing the tools, approaches and data needed to accelerate the pace of chemical risk assessment and foster incorporation of non-traditional toxicity testing data into regulatory decision-making processes. High-throughput transcriptomics (HTTr) with TempO-Seq is a promising technology for comprehensive and cost-effective bioactivity screening of chemicals. We have developed robust laboratory and bioinformatics workflows for generating HTTr data on thousands of chemicals in concentration-response. Information gleaned from these assays includes molecular point of departures representing the threshold for perturbation of cellular biology and transcriptional profiles that can be used to identify chemicals that act through similar mechanisms of action. This information would be of interest to scientists using or contemplating the use of new approach methodologies (NAMs) for next generation risk assessment (NGRA). This presentation highlight some aspects of the CCTE HTTr research program that may be of interest to the US EPA Endocrine Disruption Screening Program (EDSP).
Description:
This is a short presentation for the US EPA / Japan Ministry of the Environment 14th Bilateral Meeting on Endocrine Disruption Test Methods Development June 2021.
URLs/Downloads:
DOI: High-Throughput Transcriptomics (HTTr) Screening as a Component of NAMs-based Tiered Hazard Evaluation
2021-06-02_EDSP_US_JAPAN_HARRILL_V1.PDF (PDF, NA pp, 1630.034 KB, about PDF)