Keywords:
DRINKING WATER, BIOMARKERS OF EXPOSURE, METABOLISM, DNA ADDUCTS, DISINFECTION BYPRODUCTS.
,
Related Organizations:
Role
:OWNER
Organization Name
:UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
Mailing Address
:103 S Bldg Cb 9100
Citation
:Chapel Hill
State
:NC
Zip Code
:27599
Project Information:
Approach
:Two specific approaches will be followed towards elucidating the mechanisms
of activity of biologically potent disinfection by-products at the molecular
level:
I. Identification and Quantitation of Macromolecular Adducts Formed by
Haloacetic Acids: Structural identification and quantitation of DNA adducts will
be approached by chromatographic and mass spectrometric techniques. Quantities
of adducts sufficient for detailed structural elucidation are often difficult or
impossible to obtain by direct modification of DNA, hence direct chemical
synthesis will be undertaken to provide standards for investigation of adduct
formation in vivo.
II. Investigation of the Metabolism and Disposition of MX: Structural
identification of MX metabolites, and their tissue distribution in the rat, will
be carried out in order to gain an understanding of the nature of the active
species present at potential target organs, and the levels and duration of
internal exposure.
Cost
:$460,848.00
Research Component
:Drinking Water
Risk Paradigm
:EXPOSURE
Approach
:Two specific approaches will be followed towards elucidating the mechanisms
of activity of biologically potent disinfection by-products at the molecular
level:
I. Identification and Quantitation of Macromolecular Adducts Formed by
Haloacetic Acids: Structural identification and quantitation of DNA adducts will
be approached by chromatographic and mass spectrometric techniques. Quantities
of adducts sufficient for detailed structural elucidation are often difficult or
impossible to obtain by direct modification of DNA, hence direct chemical
synthesis will be undertaken to provide standards for investigation of adduct
formation in vivo.
II. Investigation of the Metabolism and Disposition of MX: Structural
identification of MX metabolites, and their tissue distribution in the rat, will
be carried out in order to gain an understanding of the nature of the active
species present at potential target organs, and the levels and duration of
internal exposure.
Cost
:$460,848.00
Research Component
:M/DBP (DBP)
Risk Paradigm
:RISK ASSESSMENT
Approach
:Two specific approaches will be followed towards elucidating the mechanisms
of activity of biologically potent disinfection by-products at the molecular
level:
I. Identification and Quantitation of Macromolecular Adducts Formed by
Haloacetic Acids: Structural identification and quantitation of DNA adducts will
be approached by chromatographic and mass spectrometric techniques. Quantities
of adducts sufficient for detailed structural elucidation are often difficult or
impossible to obtain by direct modification of DNA, hence direct chemical
synthesis will be undertaken to provide standards for investigation of adduct
formation in vivo.
II. Investigation of the Metabolism and Disposition of MX: Structural
identification of MX metabolites, and their tissue distribution in the rat, will
be carried out in order to gain an understanding of the nature of the active
species present at potential target organs, and the levels and duration of
internal exposure.
Cost
:$460,848.00
Research Component
:ARSENIC
Risk Paradigm
:RISK ASSESSMENT
Approach
:Two specific approaches will be followed towards elucidating the mechanisms
of activity of biologically potent disinfection by-products at the molecular
level:
I. Identification and Quantitation of Macromolecular Adducts Formed by
Haloacetic Acids: Structural identification and quantitation of DNA adducts will
be approached by chromatographic and mass spectrometric techniques. Quantities
of adducts sufficient for detailed structural elucidation are often difficult or
impossible to obtain by direct modification of DNA, hence direct chemical
synthesis will be undertaken to provide standards for investigation of adduct
formation in vivo.
II. Investigation of the Metabolism and Disposition of MX: Structural
identification of MX metabolites, and their tissue distribution in the rat, will
be carried out in order to gain an understanding of the nature of the active
species present at potential target organs, and the levels and duration of
internal exposure.
Cost
:$460,848.00
Research Component
:Drinking Water
Risk Paradigm
:RISK ASSESSMENT
Approach
:Two specific approaches will be followed towards elucidating the mechanisms
of activity of biologically potent disinfection by-products at the molecular
level:
I. Identification and Quantitation of Macromolecular Adducts Formed by
Haloacetic Acids: Structural identification and quantitation of DNA adducts will
be approached by chromatographic and mass spectrometric techniques. Quantities
of adducts sufficient for detailed structural elucidation are often difficult or
impossible to obtain by direct modification of DNA, hence direct chemical
synthesis will be undertaken to provide standards for investigation of adduct
formation in vivo.
II. Investigation of the Metabolism and Disposition of MX: Structural
identification of MX metabolites, and their tissue distribution in the rat, will
be carried out in order to gain an understanding of the nature of the active
species present at potential target organs, and the levels and duration of
internal exposure.
Cost
:$460,848.00
Research Component
:M/DBP (DBP)
Risk Paradigm
:EXPOSURE
Approach
:Two specific approaches will be followed towards elucidating the mechanisms
of activity of biologically potent disinfection by-products at the molecular
level:
I. Identification and Quantitation of Macromolecular Adducts Formed by
Haloacetic Acids: Structural identification and quantitation of DNA adducts will
be approached by chromatographic and mass spectrometric techniques. Quantities
of adducts sufficient for detailed structural elucidation are often difficult or
impossible to obtain by direct modification of DNA, hence direct chemical
synthesis will be undertaken to provide standards for investigation of adduct
formation in vivo.
II. Investigation of the Metabolism and Disposition of MX: Structural
identification of MX metabolites, and their tissue distribution in the rat, will
be carried out in order to gain an understanding of the nature of the active
species present at potential target organs, and the levels and duration of
internal exposure.
Cost
:$460,848.00
Research Component
:ARSENIC
Risk Paradigm
:EXPOSURE
Project IDs:
ID Code
:R825957
Project type
:EPA Grant