You are here:
USE OF ENZYME-LINKED IMMUNOSORBENT ASSAYS (ELISA) FOR THE DETERMINATION OF TRITON X NONIONIC DETERGENTS
Citation:
Wie, S. AND B. Hammock. USE OF ENZYME-LINKED IMMUNOSORBENT ASSAYS (ELISA) FOR THE DETERMINATION OF TRITON X NONIONIC DETERGENTS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-82/465.
Description:
An enzyme-linked immunosorbent assay (ELISA) for 4-t-octylphenyl ethoxylates such as Triton X-100 was developed. Both the 4-t-octylphenyl and the ethoxylate moiety were required for antibody recognition since members of the Triton N series showed low cross-reactivity, and polyethylene glycol polymers as well as a variety of other neutral and ionic surfactants and pesticides showed no cross-reactivity. The ELISA was sensitive in the low nanogram-per-milliliter range and was highly reproducible. It was shown to be capable of analyzing the active ingredients in vaginal contraceptives and Triton X-100 in the presence of proteins. Immunoassays thus offer advantages in the analysis of such complex mixtures.