You are here:
Cardiovascular Effects of Diesel Exhaust Inhalation: Photochemically Altered Versus Freshly Emitted in Mice
Citation:
Tong, H., J. Zavala Mendez, R. McIntosh-Kastrinsky, AND K. Sexton. Cardiovascular Effects of Diesel Exhaust Inhalation: Photochemically Altered Versus Freshly Emitted in Mice. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH - PART A: CURRENT ISSUES. Taylor & Francis, Inc., Philadelphia, PA, 82(17):944-955, (2019). https://doi.org/10.1080/15287394.2019.1671278
Impact/Purpose:
This study was conducted to compare the cardiovascular effects of diesel exhaust that was either photochemically-altered or freshly emitted. Both diesel exhausts contained particulate matter, and the fresh diesel exhaust contained NO2 and no O3, while the photochemically-altered diesel exhaust consisted of O3 and no NO2. This study demonstrates that acute inhalation to both fresh and aged diesel exhausts decreases cardiac function, with fresh diesel exhausts causing greater adverse cardiac effects, suggesting that NO2 might play an important role in diesel exhausts-induced adverse cardiovascular effects in this study.
Description:
This study was designed to compare the cardiovascular effects of inhaled photochemically altered diesel exhaust (aged DE) to freshly emitted DE (fresh DE) in female C57Bl/6 mice. Mice were exposed to either fresh DE, aged DE, or filtered air (FA) for 4 hr using an environmental irradiation chamber. Cardiac responses were assessed 8 hr after exposure utilizing Langendorff preparation with a protocol consisting of 20 min of perfusion and 20 min of ischemia followed by 2 hr of reperfusion. Cardiac function was measured by indices of left-ventricular-developed pressure (LVDP) and contractility (dP/dt) prior to ischemia. Recovery of post-ischemic LVDP was examined on reperfusion following ischemia. Fresh DE contained 460 µg/m3 of particulate matter (PM), 0.29 ppm of nitrogen dioxide (NO2) and no ozone (O3), while aged DE consisted of 330 µg/m3 of PM, 0.23 ppm O3 and no NO2. Fresh DE significantly decreased LVDP, dP/dtmax, and dP/dtmin compared to FA. Aged DE also significantly reduced LVDP and dP/dtmax. Data demonstrated that acute inhalation to either fresh or aged DE lowered LVDP and dP/dt, with a greater fall noted with fresh DE, suggesting that the composition of DE may play a key role in DE-induced adverse cardiovascular effects in female C57Bl/6 mice.
URLs/Downloads:
DOI: Cardiovascular Effects of Diesel Exhaust Inhalation: Photochemically Altered Versus Freshly Emitted in Mice