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Development of a Curated Hershberger Database
Browne, P., N. Kleinstreuer, P. Ceger, C. Deisenroth, N. Baker, K. Markey, R. Thomas, R. Judson, AND W. Casey. Development of a Curated Hershberger Database. REPRODUCTIVE TOXICOLOGY. Elsevier Science Ltd, New York, NY, 81:259-271, (2018). https://doi.org/10.1016/j.reprotox.2018.08.016
In this manuscript, we describe a Hershberger assay database compiling study results of chemicals tested in Hershberger bioassays that were conducted as part of the test guideline method development and validation, studies submitted to US EPA in response to EDSP Tier 1 test orders, and studies identified from a systematic literature review. Details on test chemical, experimental design, and experimental results were recorded, and studies under consideration were required to meet a priori criteria to ensure quality of results. The resulting database was used to characterize the variability of the Hershberger bioassay and to examine concordance of Hershberger results with other in vivo tests measuring androgen-responsive endpoints, and to then identify a set of chemicals with reproducible in vivo androgen pathway activity. This database is intended to be a scientific resource for a variety of purposes, including performance-based validation of alternatives to in vivo testing.
• A systematic literature review was conducted to identify Hershberger assays testing in vivo (anti)androgens effects of chemicals. • Details on study designs and experimental results for 134 chemicals that met pre-defined criteria were entered in a database. • Results for chemicals tested in more than one Hershberger study and Hershberger assays and other androgen-responsive in vivo assays were evaluated. • A subset of 49 chemicals were identified with effects on androgen-responsive endpoints in at least two in vivo rodent studies.
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL CENTER FOR COMPUTATIONAL TOXICOLOGY